1.
Open: File - upgrade-schema.php
Find:
add_option('comment_registration', 0);
and
Replace:
add_option('comment_registration', 1);
2.
Open: File - options-general.php
Find:
<tr valign="top">
<th scope="row"><?php _e('Membership:') ?></th>
<td> <label for="comment_registration">
<input name="comment_registration" type="checkbox" id="comment_registration" value="1" <?php checked('1', get_option('comment_registration')); ?> />
<?php _e('Users must be registered and logged in to comment') ?>
</label>
</td>
</tr>
And remove. That's all.